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Existing state-of-the-art preclinical lung models such as air–liquid interface (ALI) culture of human airway epithelial cells (hAEpCs) in transwell inserts (TWIs) or precision-cut lung slices cannot be readily scaled for rapid on-the-fly toxicity testing of the newly emerged ECs and exposure in these models often occurs by submerging the cells under e-liquid, which is not physiological as natural ALI is lost